Pci neo vector promega

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells.

construct was then cloned into the pCI-neo mammalian expres-sion vector (Promega, Madison, WI, USA) at the XholandNotl cloning sites. pCI-neo/rSP-NC1 was confirmed by direct nucle-otide sequencing at Genewiz (South Plainfield, NJ, USA).ForIB and actin bundling assay, primary Sertoli cells were transfected Vector and pFC20A/K (HaloTag® 7) T7 SP6 Flexi® Vector 4. Vectors for insect extract cell-free protein syn-thesis, such as pIX4.0 5. Mammalian expression vectors, with similar ele-ments such as pF4A CMV Flexi®, pFC14A/K (HaloTag® 7) CMV Flexi®, pFN21A/K (HaloTag® 7) CMV Flexi ®, pRL-null, pRL-CMV, pRL-SV40, pRL-TK and pCI-neo Vectors The three HiBiT CMV-neo Flexi® Vectors are configured to facilitate simple, efficient transfer of the gene of interest into a vector designed for genetic attachment of the HiBiT peptide tag to the amino or carboxy terminus of the protein of interest using the Flexi® Cloning System (Cat.# C8640).

26.03.2021 Pci neo vector promega

pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G418.

The pCI-neo Mammalian Expression Vector(a,b) carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region(b) to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. This vector can be used for transient expression or …

pCI-neo.dna. Map and Sequence File: Download Open .

Subsequently, the cytomegalovirus promoter was replaced with the cytomegalovirus promoter/chimeric intron from pCI-neo (Promega, Nacka, Sweden) using a BglII / EcoR I cloning strategy. .. The resulting vector was named as pCIn.hNGF.

pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841. pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 The pHTC HaloTag® CMV-neo Vector is a simple multiple cloning site (MCS) plasmid for traditional cloning. It is designed for use with other HaloTag® C-terminal vectors for expression of C-terminal-tagged HaloTag® fusion proteins in mammalian cells.

DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841 pCI-neo vector (Promega) that had been digested with NheI and SalI. Purification of wt and K415A / K419A 2— Sf21 cells were prepared in a 1-liter spinner at a concentration of 1 10 6 cells/ml, into which the The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G-418.

Download Free Trial Get SnapGene Viewer. Search. Your time is valuable! Mammalian pCI-neo Mammalian Expression Vector.

Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. pCI-neo Mammalian Expression Vectorは、哺乳動物細胞における安定した遺伝子発現に最適な発現ベクターです。抗生物質G418を用いてトランスフェクト細胞を選択すれば、一過性の発現または安定な発現に利用できます。 The pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. A β-globin/IgG chimeric intron located downstream of the enhancer/promoter region can further increase expression in the pCI Vector. pCI-neo. Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker.

DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841. pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient expression or for stable expression by selecting transfected cells with the antibiotic G418.

Download Name: pCI-neo. Description: Mammalian expression vector with CMV promoter, T7 and T3, chimeric intron; amp resistance (neomycin for mammalian selection); Restriction digestion of NS4B gblock and pCI-neo vector (Promega, Cat# E1841) was performed by using XhoI and XbaI restriction enzymes. Following restriction, Name: pCI-neo, Type: plasmid, Supplier: Promega. Description: The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) 5 Dec 2020 lian expression vector (pCIneo) and its derivative candidate. DNA vaccine mammalian expression vector (Promega), and pGPV-PV. (Institute プロトコル.

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pALTER®-MAX Vector. Constitutive protein expression of cloned DNA driven by the CMV promoter and expressed in many different mammalian cell types. Q5761. pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841

The resulting vector was named as pCIn.hNGF. Mammalian Expression Vector Pci Neo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Promega pgem t easy vector systems Pgem T Easy Vector Systems, supplied by Promega, used in various techniques.

Belgium). Restriction digestion of NS4B gblock and pCI-neo vector (Promega, Cat# E1841) was performed by using XhoI and XbaI restriction enzymes. Following restriction, NS4B gblock and pCI-neo vector were ligated using T4 DNA ligase (Prome ga). The ligation mix was transformed in electro-

The resulting vector was named as pCIn.hNGF. Mammalian Expression Vector Pci Neo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations.

2. pALTER®-MAX Vector. Constitutive protein expression of cloned DNA driven by the CMV promoter and expressed in many different mammalian cell types. Q5761. pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression.